Figure 9.

Effect of JAK-2 inhibitor AG-490 on LPS-induced NO production in J774 cells. J774 cells were stimulated by LPS (10 ng ml⁻¹) and treated with increasing concentrations of AG-490 (a). After 24 h incubation, nitrite concentrations in the culture medium were measured as a marker of NO production. Values are mean±s.e.m. (n=6). (b) The effect of JAK-2 inhibitor AG-490 on STAT1α nuclear translocation. J774 cells were stimulated by LPS (10 ng ml⁻¹) and treated with increasing concentrations of AG-490 for 4 h before the preparation of nuclear extracts. STAT1α translocation to the nucleus was determined by Western blotting using specific antibody against STAT1α. Chemiluminescent signal was quantified as described under the Methods section. Values are mean±s.e.m. (n=3). ^*P<0.05, ^**P<0.01 as compared with cells treated with LPS only.