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. 2006 Dec 1;34(22):6673–6683. doi: 10.1093/nar/gkl964

Table 3.

ATP hydrolysis by BACH1-WT and BACH1 variants using forked duplex as the DNA effector

Protein Keff (nM) ± SD kcat (min−1) ± SD
BACH1-WT 2.5 ± 2 25.4 ± 3.0
BACH1-M299I 4.5 ± 0.7 167 ± 15.9
BACH1-P47A — 2.11 ± 2.7

ATP hydrolysis reactions were as described under ‘Materials and Methods’ using 77 nM BACH1-WT or BACH1-M299I and 82 nM of the forked duplex effector (Table 2, DNA molecule 1) for the kcat determination. For Keff determination, 50 nM BACH1-WT or BACH1-M299I was used with the forked duplex DNA effector concentrations ranging from 0 to 75 nM.