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. 2006 Dec 1;34(22):6640–6652. doi: 10.1093/nar/gkl878

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© 2006 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Co-expression of Brn-3b with p53 increases Bax promoter activity but did not alter p21^cip1/waf1 promoter activity in ND7 cells (A) Effect of Brn-3b and p53 expression vectors on expression of the Bax promoter driving a luciferase gene reporter in ND7 cells. p53 strongly activated the Bax promoter (lane 2), but Brn-3a and Brn-3b mildly inhibited its basal activity (lanes 3 and 5) compared with the control vector (lane 1). As expected, Brn-3a repressed p53 mediated activity of the promoter (lane 4), but co-transfection of Brn-3b with p53 (lane 6) resulted in a significant enhancement in promoter activity compared with p53 alone (lane 2) (P < 0.05). Values are expressed as a percentage of the empty LTR control vectors. All values were equalized on the basis of the activity observed upon co-transfection with a control renilla expression vector. Values are the average of five experiments whose SD is shown by the bars. (B) Effect of Brn-3b and/or p53 expression vectors on the activity of the p21^CIP1/Waf1 promoter driving a luciferase reporter gene in ND7 cells. As expected, p53 alone transactivated the promoter (lane 2) and Brn-3a mildly activated the promoter (lane 3) but cooperated with p53 to enhance the promoter activity (lane 4) compared with p53 alone only (lane 2). However, Brn-3b alone had no effect on promoter activity (lane 5) but it appears to reduce p53 mediated activation on this promoter (lane 6) compared with p53 alone. Values are expressed as a percentage of the activity obtained with the empty LTR expression vector alone (which was set at 100%). All values were equalised on the basis of the activity of a control renilla reporter plasmid which was co- transfected in all cells. Values are the average of five experiments ± SD (shown by error bars). (C) The ability of Brn-3b to increase Bax promoter activity is dependent on p53 since the p53 inhibitor, α-pifitrin, prevented the transactivation seen by p53 alone (set 3) or upon co-operation of Brn-3b and p53 (set 4). The changes in promoter activity following treatment with α-pifitrin are expressed as percentage of α-pifitrin treated LTR control (set at 100%) whereas induction in the absence of α-pifitrin is expressed as percentage of untreated LTR control (also set at 100%).