Figure 3.

OAS activation by poly(A)⁺ RNA fraction of PC3 cells. (A) Activation of OAS with the OAS-selected PC3 poly(A)⁺ RNA. (B) Lack of OAS activation with non-specifically selected RNA isolated from PC3 cells on Ni²⁺-NTA beads without the addition of His₆-tagged OAS. 2-5A levels were determined by FRET assays. The same concentration of RNA (2 μg/ml) was used in (A) and (B). OAS reactions were at 37°C for 2 h.