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. 1997 Apr;65(4):1522–1526. doi: 10.1128/iai.65.4.1522-1526.1997

Expression of slime interferes with in vitro detection of host protein receptors of Staphylococcus epidermidis.

L Baldassarri 1, G Donelli 1, A Gelosia 1, A W Simpson 1, G D Christensen 1
PMCID: PMC175163  PMID: 9119497

Abstract

We hypothesized that slime may mask bacterial molecules important in the attachment of Staphylococcus epidermidis to inanimate surfaces. In support of this hypothesis, we found that slime-negative strains attached significantly better to fibrinogen or fibronectin than the parent strains and exhibited greater surface hydrophobicity. Comparable results were obtained with 53 clinical isolates.

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Selected References

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  1. Baldassarri L., Simpson W. A., Donelli G., Christensen G. D. Variable fixation of staphylococcal slime by different histochemical fixatives. Eur J Clin Microbiol Infect Dis. 1993 Nov;12(11):866–868. doi: 10.1007/BF02000411. [DOI] [PubMed] [Google Scholar]
  2. Barker L. P., Simpson W. A., Christensen G. D. Differential production of slime under aerobic and anaerobic conditions. J Clin Microbiol. 1990 Nov;28(11):2578–2579. doi: 10.1128/jcm.28.11.2578-2579.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Christensen G. D., Baddour L. M., Simpson W. A. Phenotypic variation of Staphylococcus epidermidis slime production in vitro and in vivo. Infect Immun. 1987 Dec;55(12):2870–2877. doi: 10.1128/iai.55.12.2870-2877.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Christensen G. D., Barker L. P., Mawhinney T. P., Baddour L. M., Simpson W. A. Identification of an antigenic marker of slime production for Staphylococcus epidermidis. Infect Immun. 1990 Sep;58(9):2906–2911. doi: 10.1128/iai.58.9.2906-2911.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Christensen G. D., Simpson W. A., Younger J. J., Baddour L. M., Barrett F. F., Melton D. M., Beachey E. H. Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices. J Clin Microbiol. 1985 Dec;22(6):996–1006. doi: 10.1128/jcm.22.6.996-1006.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Denyer S. P., Davies M. C., Evans J. A., Finch R. G., Smith D. G., Wilcox M. H., Williams P. Influence of carbon dioxide on the surface characteristics and adherence potential of coagulase-negative staphylococci. J Clin Microbiol. 1990 Aug;28(8):1813–1817. doi: 10.1128/jcm.28.8.1813-1817.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fleer A., Verhoef J., Pascual Hernandez A. Coagulase-negative staphylococci as nosocomial pathogens in neonates. The role of host defense, artificial devices, and bacterial hydrophobicity. Am J Med. 1986 Jun 30;80(6B):161–165. doi: 10.1016/0002-9343(86)90495-x. [DOI] [PubMed] [Google Scholar]
  8. Gatermann S., Meyer H. G. Staphylococcus saprophyticus hemagglutinin binds fibronectin. Infect Immun. 1994 Oct;62(10):4556–4563. doi: 10.1128/iai.62.10.4556-4563.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Goldmann D. A., Pier G. B. Pathogenesis of infections related to intravascular catheterization. Clin Microbiol Rev. 1993 Apr;6(2):176–192. doi: 10.1128/cmr.6.2.176. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Herrmann M., Vaudaux P. E., Pittet D., Auckenthaler R., Lew P. D., Schumacher-Perdreau F., Peters G., Waldvogel F. A. Fibronectin, fibrinogen, and laminin act as mediators of adherence of clinical staphylococcal isolates to foreign material. J Infect Dis. 1988 Oct;158(4):693–701. doi: 10.1093/infdis/158.4.693. [DOI] [PubMed] [Google Scholar]
  11. Kristinsson K. G. Adherence of staphylococci to intravascular catheters. J Med Microbiol. 1989 Apr;28(4):249–257. doi: 10.1099/00222615-28-4-249. [DOI] [PubMed] [Google Scholar]
  12. Muller E., Hübner J., Gutierrez N., Takeda S., Goldmann D. A., Pier G. B. Isolation and characterization of transposon mutants of Staphylococcus epidermidis deficient in capsular polysaccharide/adhesin and slime. Infect Immun. 1993 Feb;61(2):551–558. doi: 10.1128/iai.61.2.551-558.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Muller E., Takeda S., Goldmann D. A., Pier G. B. Blood proteins do not promote adherence of coagulase-negative staphylococci to biomaterials. Infect Immun. 1991 Sep;59(9):3323–3326. doi: 10.1128/iai.59.9.3323-3326.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pascual A., Fleer A., Westerdaal N. A., Verhoef J. Modulation of adherence of coagulase-negative staphylococci to Teflon catheters in vitro. Eur J Clin Microbiol. 1986 Oct;5(5):518–522. doi: 10.1007/BF02017694. [DOI] [PubMed] [Google Scholar]
  15. Paulsson M., Kober M., Freij-Larsson C., Stollenwerk M., Wesslén B., Ljungh A. Adhesion of staphylococci to chemically modified and native polymers, and the influence of preadsorbed fibronectin, vitronectin and fibrinogen. Biomaterials. 1993 Sep;14(11):845–853. doi: 10.1016/0142-9612(93)90006-n. [DOI] [PubMed] [Google Scholar]
  16. Paulsson M., Ljungh A., Wadström T. Rapid identification of fibronectin, vitronectin, laminin, and collagen cell surface binding proteins on coagulase-negative staphylococci by particle agglutination assays. J Clin Microbiol. 1992 Aug;30(8):2006–2012. doi: 10.1128/jcm.30.8.2006-2012.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schadow K. H., Simpson W. A., Christensen G. D. Characteristics of adherence to plastic tissue culture plates of coagulase-negative staphylococci exposed to subinhibitory concentrations of antimicrobial agents. J Infect Dis. 1988 Jan;157(1):71–77. doi: 10.1093/infdis/157.1.71. [DOI] [PubMed] [Google Scholar]
  18. Valentin-Weigand P., Timmis K. N., Chhatwal G. S. Role of fibronectin in staphylococcal colonisation of fibrin thrombi and plastic surfaces. J Med Microbiol. 1993 Feb;38(2):90–95. doi: 10.1099/00222615-38-2-90. [DOI] [PubMed] [Google Scholar]
  19. Vaudaux P., Pittet D., Haeberli A., Huggler E., Nydegger U. E., Lew D. P., Waldvogel F. A. Host factors selectively increase staphylococcal adherence on inserted catheters: a role for fibronectin and fibrinogen or fibrin. J Infect Dis. 1989 Nov;160(5):865–875. doi: 10.1093/infdis/160.5.865. [DOI] [PubMed] [Google Scholar]
  20. Yu J., Montelius M. N., Paulsson M., Gouda I., Larm O., Montelius L., Ljungh A. Adhesion of coagulase-negative staphylococci and adsorption of plasma proteins to heparinized polymer surfaces. Biomaterials. 1994 Aug;15(10):805–814. doi: 10.1016/0142-9612(94)90035-3. [DOI] [PubMed] [Google Scholar]

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