Table 3.
IL-6 release from BMDMφ in response to different stimuli
| |
No stimulus |
Zymosan |
Neisseria |
LPS |
||||
|---|---|---|---|---|---|---|---|---|
| Time (h) | Anx-A1+/+ | Anx-A1−/− | Anx-A1+/+ | Anx-A1−/− | Anx-A1+/+ | Anx-A1−/− | Anx-A1+/+ | Anx-A1−/− |
| 2 |
213±6 |
222±6 |
230±12 |
230±8 |
243±3 |
270±17 |
250±5 |
306±14* |
| 4 |
220±3 |
216±8 |
236±3 |
246±3 |
1250±51 |
3270±303* |
1350±145 |
2867±142* |
| 24 | 226±3 | 226±3 | 252±10 | 396±28 | 38,620±2895 | 43,427±3312 | 33,620±3017 | 32,383±1158 |
Adherent macrophages (0.5 × 106) prepared from Anx-A1+/+ or Anx-A1−/− mice were incubated for the reported times either alone (vehicle only), with zymosan, N. meningitidis or LPS (10 ng ml−1). At the end of the incubation period, cell-free medium was collected and assayed for IL-6 levels. Data reported are IL-6 concentrations in pg ml−1, and are expressed as mean±s.e.m. from n=3 experiments performed in duplicate.
*
P<0.05 vs respective Anx-A1 +/+ values.