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RT–PCR analysis in aortas from eNOS−/− mice. The intensity of expression of the two genes of interest (p22phox, gp91phox) was normalized to the intensity of expression of the internal standard gene GAPDH from the same aorta sample. The normalized mRNA expression of the two genes of interest from the control mice was set as 100%. *P<0.05 versus control eNOS−/−.
