Figure 7.
MB promotes NSCs differentiation into serotoninergic neurons. (a) Detection of the protein expression of 5-HT (green fluorescent), MAP2 (red fluorescent), and DAPI (blue fluorescent) in the same differentiated NSCs by using immnunofluorescent assay (bar: 20 μm). The percentages of (b) 5-HT-positive cells, (c) MAP2-positive cells, and (d) both of the 5-HT/MAP2-positive cells in the MB-treated and no MB-treated NSCs were measured by immunofluorescent signals. (b, c) The induction rates of 5-HT-positive cells and MAP2-positive cells in MB-treated NSCs. MB were significantly inhibited by PD98059. Data shown here are the mean±s.d. of three experiments. (*P<0.05, **P<0.01; MB-treated group compared to non-MB-treated group) (#P<0.05, ##P<0.01; MB- and PD98059-treated group compared to only MB-treated group). The 0.1% DMSO was used as a vehicle control.