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. 2006 May 15;148(5):619–628. doi: 10.1038/sj.bjp.0706770

Figure 4.

Figure 4

Effect of GW9508 and GW1100 on insulin secretion from the MIN6 mouse insulinoma cell line. (a) GW9508 produced a concentration-dependent increase in glucose-stimulated insulin secretion in the presence of 25 mM glucose (pEC50=6.14±0.03; **P<0.01 vs 25 mM glucose Control; n=3). No effect of GW9508 was observed in the presence of 5 mM glucose. (b) The effect of GW9508 on insulin secretion in the presence of 25 mM glucose was significantly reduced in the presence of 1 μM GW1100 (**P<0.01 vs GW9508 alone; n=3). (c) Glucose dependency of GW9508 effect on insulin secretion. Insulin secretion was significantly enhanced in the presence of GW9508 (10 μM) at higher glucose concentrations compared with lower glucose concentrations (**P<0.01; ***P<0.001; n=3). (d) Linoleic acid produced a concentration-dependent increase in glucose-stimulated insulin secretion in the presence of 25 mM glucose (*P<0.05; **P<0.01 vs 25 mM glucose Control; n=4). A significantly smaller increase in insulin secretion was observed for linoleic acid in the presence of 5 mM glucose. (e) The effect of linoleic acid on insulin secretion in the presence of 25 mM glucose was significantly reduced in the presence of 1 μM GW1100 (**P<0.01; ***P<0.001 vs linoleic acid alone; n=4). (f) GW9508 and linoleic acid effects on glucose-stimulated (25 mM) insulin secretion are additive (**P<0.01; ***P<0.001 vs linoleic acid alone; n=4). Data represent the mean±s.e.m.