Table 2.
Affinity values and kinetically derived parameters for unlabelled ligands
| Ligand | kon M−1 min−1 (k3) | koff min−1 (k4) | Kd pM (pKd) | Ki pM (pKi) |
|---|---|---|---|---|
| Atropine |
1.5±0.21 × 109 |
0.27±0.039 |
180±4 (9.71) |
9.71±0.03 |
| Clidinium |
1.1±0.07 × 108 |
0.029±0.002 |
259±7 (9.60) |
9.60±0.02 |
| Ipratopium |
4.83±0.2 × 108 |
0.07±0.005 |
160±4 (9.8) |
9.76±0.02 |
|
N-methyl scopolamine |
4.48±0.3 × 108 |
0.017±0.001 |
38±1 (10.35) |
10.39±0.03 |
| Tiotropium | 1.58±0.2 × 108 | 0.0015±0.0002 | 8±0.4 (11.1) | 11.10±0.02 |