Figure 6.

Effects of inhibitors of CaM-dependent enzymes and purified CaM kinase II on CaM-induced changes in SOC activity in inside-out patches. (a) Preincubation with 1 μM AIP prevented CaM-induced inhibition of PDBu-evoked SOC activity, whereas the excitatory effect of CaM on channel activity was not affected. (b) (i) Mean data showing that AIP prevents CaM-induced inhibition of SOC activity, but not channel excitation (n=6). (ii) and (iii) show that preincubation with cyclosporin A (n=6) and ML-9 (n=6) had no effect on CaM-evoked changes in SOC activity. (c) Bath application of 100 ngml⁻¹ CaM kinase II reversibly inhibited channel activity evoked by 100 nM CaM in an inside-out patch held at −80 mV. (d) Mean data showing CaM kinase II significantly reduced CaM-evoked channel activity (n=5, ^*P<0.05).