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Effect of endothelium on PE-stimulated NKCC1 activity in male and female rats. Endothelium-denuded aortic rings from male and female rats were preincubated with or without 10 μM bumetanide. Rings were incubated for 20 min with 10−6 M of PE (black bars) or vehicle (control, open bars). Results are the mean±s.e. of five experiments with each point assayed in triplicate; *P<0.05 and **P<0.01.
