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. 2006 Jun 19;148(8):1099–1107. doi: 10.1038/sj.bjp.0706817

Figure 7.

Figure 7

Expression of p21 and p53 in colon cancer cells after AKBA treatment. (a) Expression of p53 in HCT-116 cells treated with AKBA and vehicle control. HCT-116 cells were treated with 20 μM AKBA for 0, 24, 48 and 72 h. Expression of p53 was assayed by Western blot analysis; (b) expression of p53 in parental, p21−/− and p53−/− HCT-116 cells after treatment with AKBA. Cells were treated with 20 μM AKBA for 48 h. Expression of p53 was assayed by Western blot analysis. Control cells were treated with 0.2% ethanol; (c) changes in p21 expression in parental, p21−/− and p53−/− HCT-116 cells after treatment with AKBA. Cells were treated with 20 μM AKBA for 48 h. Expression of p21 was assayed by Western blot analysis; (d) changes in p21 expression in HT-29 cells after treatment with AKBA. HT-29 cells were treated with 30 μM AKBA for 72 h. Expression of p21 was assayed by Western blot analysis. Control cells were treated with 0.2% ethanol. Results shown are representative of three separate experiments. Expression of GAPDH was used as a housekeeping control.