Table 1.

Primer sequences and cycling conditions for semiquantitation of target gene expression

Primer	Sequence	Amplicon size (bp)	Cycling conditions
MTP-(F)	5′ GGA CTT TTT GGA CAA AAG TGA C 3′	699	94°C 40 s, 53°C 1 min, 68°C 2 min; 29 cycles
MTP-(R)	5′ GGA GAA ACG GTC ATA ATT GTG 3′
ApoC-III (F)	5′ CTC AGC TTC ATG CAG GGC TAC 3′	319	94°C 40 s, 54°C 1 min, 72°C 1 min; 26 cycles
ApoC-III (R)	5′ GGT AGG AGA GCA CTG AGA ATA CTG TC 3′
ApoA-I (F)	5′ ATG AAA GCT GCG GTG CTG ACC 3′	456	94°C 40 s, 60°C 1 min, 72°C 1 min; 21 cycles
ApoA-I (F)	5′ CAC CTT CTG GCG GTA GAG CTC 3′
ApoE (F)	5′ ACT GGC ACT GGG TCG CTT T 3′	163	94°C 40 s, 52°C 1 min, 72°C 1 min; 30 cycles
ApoE (R)	5′ GTT GTT CCT CCA GTT CCG ATT 3′
GAPDH (F)	5′ AGT CAG CCG CAT CTT CTT TTG C 3′	298	Changed according to different target genes
GAPDH (R)	5′ CTC CTG GAA GAT GGT GAT GGG A 3′