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. 2006 Jul 24;148(8):1133–1143. doi: 10.1038/sj.bjp.0706827

Figure 2.

Figure 2

Effect of forskolin on 5-HT and SB-271046-mediated cAMP formation in stably transfected HEK-293F/rat 5-HT6 cells. HEK-293F cells were stably transfected with rat 5-HT6 receptor as described in Methods. cAMP formation was determined after a 2 h serum-free incubation in the presence of either 5-HT or SB-271046 in the absence or copresence of indicated concentrations of forskolin using FlashPlate technology. pEC50 and Emax values related to 5-HT are summarized in Table 1. Data points correspond to mean values±s.e.m. from eight to ten independent experiments performed in duplicate. Specific [3H]-LSD binding (2.5–10 nM) to membranes of this cell line indicated 0.40±0.01 pmol mg protein−1. a: 5-HT, b: SB-271046.