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. 2006 Jul 24;148(8):1133–1143. doi: 10.1038/sj.bjp.0706827

Figure 5.

Figure 5

Antagonism of 5-HT-mediated cAMP formation by SB-271046 in stably transfected HEK-293F/rat 5-HT6 cells in either the absence or copresence of forskolin. HEK-293F cells were stably transfected with rat 5-HT6 receptor as described in Methods. Antagonism of 5-HT-dose-dependent cAMP formation was determined after a 2 h serum-free incubation in the presence of increasing concentrations of SB-271046 in either the absence (a, b) or copresence of 1 μM forskolin (c, d) using FlashPlate technology. Mean 5-HT dose–response curves±s.e.m. are shown from five to ten independent experiments performed in duplicate. Schild plots (b, d) were constructed using mean values±s.e.m. The gradient of the best-fit straight line was determined by linear regression. Slope (95% confidence intervals) was between 1.28–1.87 (b) and 1.42 to 2.14 (d).