Table 1.
Effect of forskolin on basal, Emax and pEC50 values of 5-HT-induced cAMP formation in stably transfected HEK-293F/rat 5-HT6 cells
| cAMP formation | |||
|---|---|---|---|
| Basal | 5-HT | ||
| (pmol 106 cells−1) | Emax (pmol 106 cells−1) | pEC50 | |
| w/o Forskolin | 4.6±0.4 | 15.2±0.4 | 6.98±0.08 |
| 1 μM Forskolin | 6.4±0.4a | 26.1±0.5b | 8.06±0.08c |
| 3 μM Forskolin | 8.8±0.7 | 26.8±0.5 | 8.22±0.09 |
| 10 μM Forskolin | 12.7±0.8 | 27.3±0.6 | 8.60±0.13d,e |
Emax and pEC50 values were derived from cAMP-mediated 5-HT response curves as illustrated in Figure 1a. Data correspond to mean±s.e.m. values of eight to ten independent experiments performed in duplicate. a: P<0.05 versus basal cAMP level w/o forskolin; b: P<0.001 versus Emax 5-HT w/o forskolin; c: P<0.001 versus pEC50 5-HT w/o forskolin; d: P<0.001 versus pEC50 5-HT 1 μM forskolin; e: P<0.05 versus Emax 5-HT 3 μM forskolin.