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FOXJ1CreER2T transgene efficiently lineage-labels ciliated cells after tamoxifen injection. (A) FOXJ1CreER2T transgene construct. (B–E) X-gal-stained (blue) FOXJ1-CreER2T; Rosa26R adult airways after tamoxifen injection. (B) Whole-mount ventral trachea. (C) Intralobular airway. (D and E) Sections of anti-β-tubulin-stained trachea (brown) (D) and distal bronchiole (E). Most ciliated cells but no Clara cells (arrows) are lineage-labeled. (Scale bars: B and D, 200 μm; C and E, 20 μm.)
