Skip to main content
. Author manuscript; available in PMC: 2006 Dec 28.
Published in final edited form as: Biochemistry. 2005 Mar 1;44(8):3074–3081. doi: 10.1021/bi047716h

Table 1.

Apparent Steady-State Kinetic Parameters for His6-Tagged MTOX at pH 8.0a

N-methyltryptophan sarcosine
kcat (min−1) 1190 ± 50    23 ± 7 
Km (mM) 0.25 ± 0.02 44 ± 5 
kcat/Ksarc (mM−1 min−1) 4700 ± 200  0.51 ± 0.04
a

Air-saturated 0.1 M potassium phosphate and 1 mM EDTA at pH 8.0 and 25 °C.