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Kinetic Parameters for Sarcosine Oxidation by MTOX at pH 9.4a
| model (equation) | kcat (min−1) | KO2 (μM) | kcat/KO2 (min−1 μM−1) | Ksarc (mM) | kcat/Ksarc (min−1 μM−1) | Ki,sarc (mM) | χ 2 |
|---|---|---|---|---|---|---|---|
| sequential (1) | 251 ± 13 | 5 ± 3 | 50 ± 30 | 100 ± 14 | 2.5 ± 0.4 | 130 ± 160 | 1724 |
| ping-pong (2) | 262 ± 12 | 8 ± 2 | 33 ± 8 | 114 ± 10 | 2.3 ± 0.2 | NA | 1839 |
| rapid equilibrium (3) | 233 ± 8 | 16 ± 5 | 15 ± 5 | 83 ± 9 | 2.8 ± 0.8 | NA | 1964 |
0.1 M ACES, 52 mM Tris, 52 mM ethanolamine-HCl, and 1 M KCl at pH 9.4.
