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Deuterium Kinetic Isotope Effects on Sarcosine Oxidation by MTOX
| pH | kinetic isotope effect | pH | kinetic isotope effect |
|---|---|---|---|
| 8.0a,b | 6.8 ± 0.6 | 8.0d,e | 7.4 ± 0.5 |
| 9.4a,c | 6.6 ± 0.4 | 9.4c,d | 7.7 ± 0.3 |
| 10.0a,c | 7.4 ± 0.4 |
From steady-state kinetic analyses.
0.25 M potassium phosphate.
0.1 M ACES, 52 mM Tris, 52 mM ethanolamine-HCl, and 1 M KCl.
From anaerobic stopped-flow analyses with 250 mM sarcosine.
0.1 M Tris and 0.5 M KCl.
