Table I.
Pre-steady-state kinetic parameters of HIV-1 and MuLV RTs with four dNTPs
| DNTPs | RT | Kinetics parameters (fold differences)a |
||
|---|---|---|---|---|
| kpol | Kd | Kpol/Kd | ||
| s−1 | μm | μm−1 s−1 | ||
| DCTP | HIV-1 | 37.5 ± 7.5 | 0.334 ± 0.4 | 112.5 |
| MuLV | 47.2 ± 8.1 (×1.3) | 18.1 ± 9.4 (×54.2) | 2.6 (×43.3) | |
| DATP | HIV-1 | 33.7 ± 0.6 | 0.610 ± 0.5 | 55.3 |
| MuLV | 159.2 ± 4.7 (×4.7) | 74.2 ± 1.2 (×121.6) | 2.1 (×26.3) | |
| DGTP | HIV-1 | 39.4 ± 5.6 | 3.9 ± 0.04 | 10.2 |
| MuLV | 72.2 ± 3.8 (×1.8) | 25.2 ± 8.3 (×6.5) | 2.9 (×3.5) | |
| DTTP | HIV-1 | 36.6 ± 7.5 | 2.5 ± 1.4 | 14.6 |
| MuLV | 159.3 ± 14.9 (×4.4) | 115.9 ± 9.3 (×46.4) | 1.4 (×10.4) | |
| DTTPb | HIV-1c | 21.7 ± 0.7 | 1.1 ± 0.1 | 19.7 |
| MuLV | 20.0 ± 0.6 (×1.1) | 44.2 ± 4.0 (×40.2) | 0.45 (×43.8) | |
a
Fold differences of MuLV RT relative to HIV-1 RT.
b
dTTP incorporation with the 16-mer matched T primer.
c
Data published previously (39).