Table II.
Pre-steady-state kinetic parameters of HIV-1 and MuLV RTs for misincorporation
| dNTP | RT | Kinetic parameters (fold differences)a |
|||
|---|---|---|---|---|---|
| kpol | Kd | kpol/Kd | Misinsertion fidelityb | ||
| s−1 | μm | μm−1 s−1 | |||
| DTTP (correct) | HIV-1c | 21.7 ± 0.7 | 1.1 ± 0.1 | 19.7 | |
| MuLV | 20.0 ± 0.6 (×1.1) | 44.2 ± 4.0 (×40.2) | 0.45 (×43.8) | ||
| DGTP (incorrect) | HIV-1c | 0.037 ± 0.001 | 13.8 ± 2.2 | 2.7 × 10−3 | 7296.3 |
| MuLV | 0.020 ± 0.002 (×1.9) | 1178.3 ± 169.2 (×85.4) | 1.7 × 10−5 (×158.8) | 26470.6 (×3.6) | |
| DCTP (incorrect) | HIV-1c | 0.027 ± 0.001 | 117.3 ± 7.3 | 2.3 × 10−4 | 85652.2 |
| MuLV | 0.032 ± 0.002 (×1.2) | 1546.5 ± 176.7 (×13.2) | 2.1 × 10−5 (×10.9) | 21428.6 (×0.25) | |
a
Fold differences of MuLV RT relative to HIV-1 RT.
b
(kpol/Kd)correct/(kpol/Kd)incorrect.
c
Data published previously (39).