Table III.

Pre-steady state kinetic parameters of dTTP incorporation by HIV-1 and MuLV RTs and binding affinity of MuLV and HIV-1 RTs for mismatched T/P

RT	kpol	Kd	kpol/Kd	Mismatch extension fidelitya	Binding affinity (Kd) to mismatched T/Pb
	s−1	μm	μm−1 s−1		nm
HIV-1c	0.5 ± 5.6 × 10−3	14.1 ± 0.6	0.04	493.5	53.8 ± 2.8
MuLV	11.6 × 10−3 ± 0.1 × 10−3 (×43.1)d	39.4 ± 2.3 (×2.8)d	2.9 × 10−4 (×137.9)d	1552.7 (×3.1)d	203.2 ± 7.6 (×3.8)

^a(k_pol/K_d)_matched/(k_pol/K_d)_mismatched.

^bK_d values were obtained using Eq. 3 as described previously (48).

^cData published previously (39).

^dFold difference in fidelity relative to wild-type HIV-1 RT.