Table 1.
Primers designed with Primer Premier 5.0 for amplifying the complete mitochondrial genome of U.thibetanus mupinensis
| No. | Forward Primer | Reverse Primer | Product length (bp) | Forward Primer Sequence (5'-3') | Reverse Primer Sequence (5'-3') |
|---|---|---|---|---|---|
| 1 | 1-25 | 1072-1095 | 1095 | GATCACACATAACTGTGGTGTCATG | CGGAGACTTACATGTGTAATCTTG |
| 2 | 1036-1059 | 3622-3646 | 2611 | TAAAGGTTTGGTCCTAGCCTTCCC | AAGCCCTGTCTCTTGGGCAGTATTG |
| 3 | 2857-2882 | 3824-3849 | 993 | AGATTAAAAGAAGTAAAAGGAACTCG | GGCCCTACAATGTTTGGTCCTTTACG |
| 4 | 3725-3752 | 4657-4681 | 957 | ATGTTTATAATTAACACTATCTCACTAG | TTATATTTGGGGGGGAATGCTTGCT |
| 5 | 4181-4205 | 5207-5230 | 1050 | GTCCTACTAATGAATGGCTCATTCG | CAGAAGTGGAAGGGGGATAGGCCG |
| 6 | 4852-4878 | 5928-5953 | 1101 | ATACCCCGAAAATGTTGGTTTATCCCC | GGTCTTTTTAGCCTAAATCTCTAGTC |
| 7 | 5560-5583 | 6487-6514 | 955 | CCACAACAACACTATCACTGTCCC | GATTATCACGAATGCATGGGCAGTTACG |
| 8 | 6286-6310 | 7834-7854 | 1568 | CAGTCTAGTGCTTTTATCAGCCATT | AGTATAACGTAAGCGGGTTCT |
| 9 | 7202-7224 | 8188-8209 | 1008 | GGTATAGATGTCGACACACGAGC | GCCGGCAAGATGGTTCATACCG |
| 10 | 7958-7981 | 8728-8748 | 791 | CTTTGTCAGGGTTAAATTATAGGT | GGAGAAGTCTGCATTCTCAGT |
| 11 | 8671-8694 | 9588-9612 | 942 | CCTCAATACTATAAAATCATTGAG | GTTTGGTGAGTCATTAGGTGTTATC |
| 12 | 9588-9610 | 10434-10457 | 870 | GATAACACCTAATGACTCACCAA | GTTGATTGTTTCTTTCTGGATTAC |
| 13 | 10405-10427 | 11150-11178 | 774 | CAATTGACTTCCAATCAATTAGC | ATTTTTAGCATTGTAAGAG |
| 14 | 11147-11172 | 12522-12548 | 1402 | AATCTCTTACAATGCTAAAAATTATC | AAACTATATTTACAGTAAATGGGCCCC |
| 15 | 12196-12215 | 12947-12971 | 776 | GGTCTACAAACACTCCTTCC | AGCTTAGAGTTAGCTTTAGGGTTTG |
| 16 | 12703-12722 | 15134-15158 | 2456 | CAAAAAATTGGTGCAACTCC | GTTTTTCGGATGTTGGTCATTAAGG |
| 17 | 15139-15161 | 16261-16278 | 1140 | ATGACCAACATCCGAAAAACCCA | TCTTCATTTTGAGAGGTT |
| 18 | 16194-16215 | 158-183 | 858 | GCTAGCCTCCATCCTCTACTTC | CCCGTAACCATTGACTGAATAGCCCC |