Table 2.
Reaction condition of PCR for 18 pairs primerse (Group 1 primers to have: 2, 5, 7, 9, 18; Group 2 primers to have: 1, 3, 4, 6, 8, 10,12,15; Group 3 primers to have: 11,14,16; Group 4 primers to have: 13,17)
| Reaction condition | Group 1 | Group 2 | Group 3 | Group 4 |
|---|---|---|---|---|
| Denaturation temperature | 94℃ | 94℃ | 94℃ | 94℃ |
| Time | 5min | 5min | 5min | 5min |
| Cycleindex | 1 | 1 | 1 | 1 |
| Denaturation temperature | 94℃ | 94℃ | 94℃ | 94℃ |
| Time | 30s | 30s | 30s | 30s |
| Annealing temperature | 60-50℃ | 58-48℃ | 55-46℃ | 49-40℃ |
| Time | 30s | 30s | 30s | 30s |
| Primer extension temperature | 72℃ | 72℃ | 72℃ | 72℃ |
| Time | 90s | 90s | 90s | 90s |
| Cycleindex for each annealing temperature | 2 | 2 | 2 | 2 |
| Denaturation temperature | 94℃ | 94℃ | 94℃ | 94℃ |
| Time | 30s | 30s | 30s | 30s |
| Annealing temperature | 55℃ | 52℃ | 49℃ | 45℃ |
| Time | 30s | 30s | 30s | 30s |
| Primer extension temperature | 72℃ | 72℃ | 72℃ | 72℃ |
| Time | 90s | 90s | 90s | 90s |
| Cycleindex | 30 | 30 | 30 | 30 |
| Preservation temperature | 4℃ | 4℃ | 4℃ | 4℃ |