Figure 1  .

Detection of (A) ospA and (B) p66 gene sequences of B burgdorferi by PCR in SF and SM from patient 4. Nested PCR products were visualised on a 3% agarose gel stained with ethidium bromide. At presentation in our clinic, PCR was positive in SF (lane 3). After antibiotic therapy, PCR became negative in SF (lane 5) but was positive in SM (lane 7). Lane M: molecular weight markers, lane 1: positive control (B burgdorferi sensu stricto, strain LW2), lanes 3, 4, 6: negative control (water lane).