Figure 4  .

Lectin competition. HeLa protein western blot. Lane 1 incubated with anti-p68 negative control serum, 2 with anti-p68 positive RA serum, 3 with anti-p68 positive rabbit serum; 4 + 5 with DBA and 6 +7 with WGA over night. Subsequent incubation with anti-p68 positive RA serum (5 + 7) and with anti-p68 positive rabbit serum (4 + 6). Detection with FITC conjugated secondary antibody. The lectin WGA, but not DBA competed with the RA serum anti-p68 antibody for binding to p68 (7, 5). Neither lectin could compete with the rabbit anti-p68 antibody (6, 4).