Figure 1.

The initial DNA bubble-duplex construct used and the RNA products obtained. The structure and sequence of the RNA primer and the DNA bubble-duplex construct used in this work are shown. The direction of synthesis is indicated by an arrow. On addition of RNA polymerase and Mg²⁺/NTPs, three different transcription products are obtained. Product 1 corresponds to a partially hybridized RNA transcript, Product 2 to a fully hybridized transcript, and Product 3 to a fully released transcript (see text).