Figure 5  .

Mobility shift assay of IRP (A) and NFκB (B) from synovial fluid mononuclear and polymorphonuclear cells from a representative case of three RA patients. In panel A, lanes 1 and 2 correspond to active IRP in synovial fluid mononuclear cells and lanes 3 and 4 to active IRP in synovial fluid polymorphonuclear cells. Samples in lanes 1 and 3 were treated with 2-mercaptoethanol (2-ME) to convert inactive IRP to the active form (see methods). In panel B, lane 1 corresponds to NFκB activity in synovial fluid mononuclear cells and lane 2 to NFκB activity in synovial fluid polymorphonuclear cells.