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No effect of MTX on LDH release from cultured cartilage. Bovine articular cartilage was incubated for two days in the presence of MTX (0 to 10 µM), and the amount of lactate dehydrogenase (LDH) released into the culture medium determined fluorometrically. The detergent Triton-X 100 (TX) was used as a positive control. Bars represent means and SEM of eight cartilage explants per group.
