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No inhibition by MTX of chondrocyte protein synthesis in vitro. Bovine articular cartilage was incubated for two days in the presence of MTX (0 to 100 µM), or cycloheximide (CHX) (10 mg/l). The samples were subsequently labelled with [35S]-methionine for four hours, hydrolysed with papain, and incorporation of the tracer into trichloroacetic acid precipitable macromolecules was determined. Bars represent means and SEM of eight cartilage explants per group.
