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DNA fragmentation of ceramide treated RA synovial cells. RA synovial cells were treated with 10 µM C2-ceramide for the indicated periods. Extracted DNA was electrophoresed on a 2% agarose gel, and stained with ethidium bromide. Lane 1:1kb ladder DNA molecular weight marker, lane 2:0.1% ethanol, lane 3:C2-ceramide for 24 hours, lane 4:C2-ceramide for 48 hours.
