Figure 3  .

(A) Neutrophil adhesion to HUVEC and SMEC monolayers pre-incubated with TNFα (0-100 IU/ml). Results show mean binding of neutrophils as a percentage of the initial number added and depict 95% confidence intervals for the mean binding. SMEC occasionally, but not consistently, bind significantly (p<0.01**) higher numbers of neutrophils than HUVEC (n=5). (B) T lymphocyte adhesion to HUVEC and SMEC monolayers pre-incubated with TNFα (0-100 IU/ml). Results are expressed as the mean percentage binding of the original number of lymphocytes added and depict 95% confidence intervals for the mean binding. No significant differences were observed between cell types (n=5). (C) Comparison of static PMN adhesion to TNFα (0-100 IU/ml) stimulated endothelial cell monolayers. Results show mean binding of neutrophils as a percentage of the initial number added and depict 95% confidence intervals for the mean binding. Results indicate differences in neutrophil binding to the two endothelial cell types before the addition of anti-ICAM-1 antibody (50 µg/ml), rarely reaches significance (p<0.05*). However, in the presence of anti-ICAM-1 antibody SMEC are capable of binding significantly (p<0.05*, p<0.01**, p<0.001***) more PMN than similarly treated HUVEC at a number of TNFα concentrations (n=3).