Figure 4  .

Adhesion of (A) peripheral blood neutrophils and (B) T lymphocytes to SMEC and HUVEC monolayers pre-stimulated with TNFα (50 IU/ml) for five hours. The cell counts represents the mean number of leucocytes (per mm²) attached to the endothelial monolayers, after 30 minutes of continuous circulation, at points in the flow chamber where wall shear rates were calculated at 2.4 and 4.0 dynes/cm² respectively and depict 95% confidence intervals for the mean response. SMEC monolayers bound significantly (p<0.05*) higher numbers of both neutrophils at both 2.4 and 4.0 dynes/cm² in comparison with HUVEC at the same rates of shear. Differences in T lymphocytes adhesion to SMEC in comparison with HUVEC were only significant (p<0.05*) at higher (4.0 dynes/cm²) rates of shear (n=3). (C) Immunohistochemical analysis of adherent T lymphocyte phenotype and initial proportions in healthy peripheral blood. Results are expressed as the proportion of cells bearing a CD45RO+ phenotype as a percentage of the total and depict 95% confidence intervals for the mean response. Cell populations adherent to both SMEC and HUVEC monolayers at both 2.4 and 4.0 dynes/cm² display a significant (p<0.01**) increase in the proportions of CD45RO+ cells in comparison with peripheral blood. SMEC also display a significantly (p<0.05*) increased capacity to bind such cells in comparison to HUVEC at higher (4.0 dynes/cm²) rates of shear (n=3).