Figure 2  .

Esculetin (EST) inhibits collagen loss from bovine nasal cartilage stimulated to resorb with interleukin 1α (IL1α) and oncostatin M (OSM). Bovine nasal cartilage discs were cultured for 14 days with IL1α (1 ng/ml) in combination with OSM (10 ng/ml) ± EST (10-330 µmol/l). Media were harvested and replenished at day 7. Collagen release is expressed as a percentage of the total present in the explant. The cumulative collagen loss over 14 days of culture is presented. Results are expressed as mean (SD) (n=4). Statistical analyses compared collagen loss seen with IL1α + OSM alone to that seen with IL1α + OSM + EST. ***p<0.001.