Figure 3  .

Esculetin (EST) inhibits proteoglycan loss from bovine nasal cartilage. Bovine nasal cartilage discs were incubated for 14 days with interleukin 1α (IL1α) and oncostatin M (OSM) at the concentrations indicated ± EST (100 µmol/l). Media were harvested and replenished at day 7; media and cartilage were harvested at day 14. Proteoglycan loss is expressed as a percentage of the total present in each explant; the data illustrated are for proteoglycan loss by day 7 of culture. Results are mean (SD) (n=4). Statistical analyses compared proteoglycan loss seen with IL1α + OSM treatment to that seen with IL1α + OSM + EST for each set of cytokine concentrations as indicated by the bars. ***p<0.001, *p<0.05.