Figure 8  .

Esculetin (EST) inhibits matrix metalloproteinase-1 (MMP-1) promoter activity. T/C28a4 cells were transiently transfected with an MMP-1/luciferase promoter construct. Two days after transfection, cells were incubated with interleukin 1α (IL1α; 1 ng/ml) and/or OSM (10 ng/ml) ± EST (100 µmol/l) for 24 hours. Cells were then harvested and luciferase activities measured, and normalised to the amount of DNA plasmid in each well. The results represent the mean (SD) (n=3) of a representative experiment performed three times.