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Activation of Hsk1 kinase by recombinant Dfp1. Increasing amounts (0, 10, 25, or 50 ng) of GST-Dfp1 were added to purified monomeric Hsk1 (≈5 ng, lanes 4–7). Proteins were incubated under kinase assay conditions. All reactions contained 100 ng of GST-Cdc19 substrate. Control reactions contained no added protein (lane 1), GST-Dfp1 (50 ng) alone (lane 2), GST (100 ng) with Hsk1 monomer (lane 3), or heterodimeric Hsk1⋅Dfp1 (lane 8).
