Figure 2.

Deletion of dfp1⁺ delays entry into S phase. (A) Tetrad analysis of the diploid dfp1⁺/dfp1∷ura4⁺ disruption strain GBY405. (B) Representative microcolonies from one tetrad from GBY405. Microcolonies (Upper Right and Lower Left) were uracil auxotrophs and, therefore, dfp1⁺. Germinated spores (Upper Left and Lower Right) did not give rise to colonies. (C) DNA contents from flow cytometry of germinating spores of GBY405. Spores were germinated in medium containing uracil (+ura) to permit germination of all spores or in medium lacking uracil (−ura) to allow germination of spores bearing the dfp1 disruption only. The positions of 1C and 2C DNA contents are indicated. (D) Depletion of Dfp1. Expression of dfp1 in the strain GBY429 was repressed by the addition of thiamine to 5 μg/ml at t = 0 hr. DNA content was measured by flow cytometry every hour after repression.