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Autoradiogram of Southern analysis of replicated (DpnI-resistant) viral DNAs from a transient replication assay with HPV 31 wild type (WT) and E7 mutants in SCC13 cells. Transfections of viral DNAs and Southern analysis were carried out as described in the legend to Fig. 2. DNA standards (Stds) are shown on the left and contain 500, 25, 2.5, and 0.5 pg of linearized wild-type HPV 31 DNA.
