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Autoradiogram of Southern analysis of stably transfected HFKs with HPV 31 wild type (WT) and E7 mutants. Total genomic DNAs were prepared and digested as described in Materials and Methods. The migration of the various DNA forms is indicated on the left. These experiments were repeated with three independent transfections, and each yielded similar results. The wild-type sample (lane 1) is the same sample shown in Fig. 3, lane 1.
