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. 2001 May;60(5):473–482. doi: 10.1136/ard.60.5.473

Figure 5  .

Figure 5  

Effect of interferon γ (IFNγ) on human salivary gland duct (HSG) CD80 or CD86 expression as measured by immunoperoxidase cell ELISA. Cells were cultured and stimulated with IFNγ. (A) CD80 expression after 24 hours' exposure to increasing concentrations of IFNγ; (B) CD86 expression after 24 hours' exposure to increasing concentrations of IFNγ; (C) time course of induction of CD80 by IFNγ (100 U/ml); (D) time course of induction of CD86 by IFNγ (100 U/ml); (E, F) effect of blockade of CD80 (E) and CD86 (F) expression by anti-IFNγ. HSG cells were cultured and stimulated with 100 U/ml of IFNγ and with 0.01, 0.1, and 1 µg/ml of monoclonal anti-IFNγ antibody (R&D). Addition of 1 µg/ml of anti-IFNγ inhibited the up regulation of IFNγ. All values are expressed as means (SD) of triplicate wells. *p<0.05, **p<0.01 indicate significant difference from the control (untreated) OD. Absence of an error bar indicates that the error was less than the symbol size.