Figure 7  .

Effect of interferon γ (IFNγ) in CD80 or CD86 expression on renal cortical epithelial (HRCE) cells as measured by immunoperoxidase cell ELISA. Cells were cultured and stimulated with IFNγ. (A) CD86 expression after 12 hours' exposure to increasing concentrations of IFNγ; (B) CD80 expression was not induced by 12 hours' exposure to IFNγ; (C) Time course of induction of CD86 by IFNγ (100 U/ml); (D) effect of blockade of CD86 expression by anti-IFNγ (a-IFN). HRCE cells were cultured and stimulated with 100 U/ml of IFNγ and 1 µg/ml polyclonal anti-IFNγ (Genzyme). Addition of 1 µg/ml of anti-IFNγ inhibited the up regulation of IFNγ. All values are expressed as means (SD) of triplicate wells. *p<0.05, **p<0.01 indicate a significant difference from control (untreated) OD. No error bar indicates that the error was less than the symbol size.