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Recombinant SSE1 is active as both structure-specific endonuclease and RNase H. (A) Substrate for endonuclease assays. The 5′ 32P label is indicated by ∗. (B) Endonuclease assays with recombinant SSE1. Lane 1, no enzyme added; lane 2, 0.5 ng of enzyme; lane 3, 0.1 ng of enzyme; lane 4, 0.05 ng of enzyme; 21-nt product is indicated by an arrow. The size of the 21-nt product was determined by comparison to a sequencing ladder as in ref. 15. (C) RNase H assays with recombinant SSE1.
