Figure 4 .

Effect of 1α,25(OH)₂D₃ supplementation on the number of TRAP positive MNCs. In the co-culture of NA-BMCs and granulation fibroblasts in the presence of 1α,25(OH)₂D₃ and ht ROSCM or in the presence of just ht ROSCM, TRAP positive MNCs appeared at two days and the number of TRAP positive MNCs increased until four days, reaching a maximum, before decreasing with time until the end of the co-culture. There was no significant difference in the number of TRAP positive MNCs (in the presence of just ht ROSCM compared with the presence of 1α,25(OH)₂D₃ and ht ROSCM).