Figure 2 .
Cell type-specific mRNA expression of collagenase 3 in the synovial membrane in RA. Collagenase 3 mRNA was detected by in situ hybridisation (NBT/BCIP colour reagent; black) and expressed in fibroblast-like cells (arrowheads) of the synovial membrane which were immunohistochemically labelled with mAb D7-Fib (APAAP method; red) known to recognise fibroblasts (A). Lymphocytic infiltrates (L) in the synovial membrane were negative for collagenase 3 mRNA expression (B). Scale bar 80 µm in A; 130 µm in B.