Figure 3 .

mRNA expression of collagenase 3, MT1-MMP, and gelatinase A in the synovial membrane in RA. Northern blot analysis was performed using total RNA from six separate synovial membrane specimens of patients with RA by successive hybridisation with radioactively labelled cDNA probes corresponding to collagenase 3, MT1-MMP, and gelatinase A, as indicated on the left. The mRNA expression levels of MT1-MMP and gelatinase A were only slightly raised in patients with RA expressing collagenase 3 mRNA in the synovial membrane (lanes 1 and 2) compared with patients without collagenase 3 mRNA expression (lane 3–6). GAPDH was used to check the loading of RNA.