Figure 5 .

Cell type-specific mRNA expression of MT1-MMP and gelatinase A in the synovial membrane in RA. MT1-MMP and gelatinase A mRNA were detected by in situ hybridisation (NBT/BCIP colour reagent; black). Both MMPs are expressed in fibroblast-like cells (arrowheads) of the synovial membrane, which were immunohistochemically labelled with mAb D7-Fib (APAAP method; red) as shown in (A) and (B), respectively. MT1-MMP mRNA expression (black) was detected in endothelial cells (arrows) marked with an mAb against type IV collagen of the basal membrane (red, arrowheads) (C). Scale bar 80 µm in A and B; 200 µm C.