Vγ TCR gene amplification in PBL. Each lane represents hybridisation with a family specific Vγ probe, after amplification with a family specific 5` Vγ primer and a 3` Cγ primer. Five different Vγ primers (detecting Vγ1.2 in lane 1, Vγ1.2 and 1.4 in lane 2, Vγ1–1.8 in lane 3, Vγ1.8 in lane 4, and Vγ2 in lane 5) were used.